Phase contrast microscopy is a type of light microscopy that is used to visualize transparent specimens that are difficult to see with other types of microscopes. This technique works by taking advantage of the fact that different parts of a specimen will cause light waves to be shifted by different amounts. By using a special phase contrast microscope, it is possible to create an image that highlights these differences in phase.
The regulation of phase contrast microscopy is critical for obtaining high-quality images. This regulation is achieved through careful alignment of the microscope's components and proper adjustment of the microscope's settings. When this regulation is not properly maintained, images can be blurry or distorted.